The Super Eagles will take on Ukraine in a friendly in Kiev on September 10, according to officials.
President of the Nigeria Football Federation (NFF), Amaju Pinnick, disclosed the game will expose more players to international football.
Nigeria are 33rd team in the world by the latest FIFA Rankings, while Ukraine are 25th.
Three-time African champions Nigeria placed third at the recent AFCON in Egypt under Franco-German coach Gernot Rohr, who still has a year to run on his contract.
The Eagles begin their qualifying campaign for the 2021 AFCON on November 11 against Benin Republic at the southern Nigerian town of Benin City.
Sierra Leone and Lesotho are the other teams in their qualifying group.
The top two teams qualify for the final tournament in Cameroon.