Captain Asisat Oshoala will not feature for the Super Falcons in two friendlies against Canada.
No reason was given for the absence of the Barcelona Femeni star.
Other notable absentees are China-based striker Desire Oparanozie and Patricia George.
Some of the invited players have already withdrawn from the squad due to injury and visa hitches.
Latest additions to the Super Falcons, Ashliegh Plumptre, Ifeoma Onumonu and Esther Okoronkwo, who shone in the WAFCON playoffs against Cote d’Ivoire, are listed on the 25-person squad.
The first match against the Olympic Gold medalists will be on Friday and Monday, April 11 in Canada but will kick off at 7.30pm Nigeria time (3.30am Vancouver time on 9 th April and 12 th April respectively).
The last time Nigeria and Canada battled each other was in the build up to the France 2019 FIFA Women’s World Cup and the Super Falcons fell 2-1 to the Canadians despite Oparanozie’s effort off Kanu’s assist in Pinatar, Spain.
And Friday’s clash at the BC Place in Vancouver is another date for both teams after many years, following their epic 3-3 draw at the 1995 FIFA Women’s World Cup finals in Sweden.
The fixtures between the world ranked no. 6 and 39 nations are billed to get the reigning African champions prepared for their 2022 Women’s Africa Cup of Nations campaign which will be held from July 2 to 23 in Morocco.
The full squad:
Goalkeepers: Chiamaka Nnadozie [Paris FC], Tochukwu Oluehi [Maccabi Kishronot Hadera]
Defenders: Ashliegh Plumptre [Leciester City], Onome Ebi [Unattached], Osinachi Ohale [Alaves], Nicole Payne [West Virginia Mountaineers], Michelle Alozie [Houston Dash], Akudo Ogbonna [Edo Queens], Glory Ogbonna [Santa Teresa CD]
Midfielders: Christy Ucheibe [SL Benfica], Peach Efih [Maccabi Kiryat Gat], Amanda Uju, Rasheedat Ajibade [Atletico Madrid], Toni Payne [Sevilla], Regina Otu [Minsk], Ngozi Sonia Okobi-Okeoghene [Eskilstuna United], Rita Chikwelu [Madrid SBB], Halimatu Ayinde [Eskilstuna United]
Strikers: Esther Okoronkwo [Saint Ettiene], Vivian Ikekwukwu [Gintra], Ifeoma Onumonu [Gotham FC], Uchenna Kanu [UNAL Tigers], Francisca Ordega [CSKA Moscow]