Emmanuel Dennis will not be part of the Super Eagles who will battle Algeria in a series of friendlies, so too Samuel Chukwueze.
However, Portugal-based forward Saviour Godwin and Rivers United leftback Ebube Duru have been handed late call-ups by the Eagles.
Nottingham Forest winger Dennis was drafted to the squad after Turkey-based Henry Onyekuru suffered an injury.
But he has also now dropped out.
Saviour will get his chance with the Super Eagles after he has been capped at U17, U20 and U23 levels by Nigeria.
He has been outstanding for Portuguese club Casa Pia after he helped them win promotion to the top flight this season.
Ebube Duru from NPFL champions Rivers United will now be the only home-based player in this party following a late invitation.
In the meantime, 18 players have already hit Constantine for the friendlies.
Nigeria will play hosts Algeria first on Friday in Constantine, before they play a second match in Oran on September 27.